WIDINATHA, ARYA and Hartayanie, Laksmi and Lindayani, - (2013) ISOLATION AND CHARACTERIZATION OF LACTIC ACID BACTERIA PRODUCING ANTIMICROBIAL COMPOUNDS FROM SMALL INTESTINE OF CHICKEN. In: The 4 th International Conference of Indonesian Society Lactic Acid Bacteria (ISLAB), 25 January 2013, Yogyakarta.
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Abstract
In food industry, Lactic Acid Bacteria (LAB) have important role for food preservation because of the ability of some strains to produce antimicrobial compounds, including organic acids, hydrogen peroxide and bacteriocins. A number of studies have reported that the LAB have antagonist properties by inhibit the growth of enteric pathogens i.e. Staphylococcus aureus, Escherichia coli, Listeria monocytogenes, and Salmonella sp. Lactic acid bacteria are commonly found in the gastrointestinal tract of various endothermic animals and humans. The aim of this research was to isolate and to characterize lactic acid bacteria from small intestine of chickens that have antimicrobial activity against enteric pathogenic i.e. Staphylococcus aureus and Escherichia coli bacteria. Three steps in this research were LAB isolation, characterization and antimicrobial activity testing using diffusion method. Nineteen strains were isolated and identified as lactic acid bacteria based on their morphology and biochemical characteristics. All isolates were Gram-positive, non-sporulating, non-motile, catalase negative, did not produce gas and classified as Lactobacillus and Enterococcus. In antimicrobial testing, all isolates produced antimicrobial compounds which were active against pathogenic bacteria, indicated by the formation of clear zones around the well (9-15 mm diameter zones). The result show that isolate number 411 had the highest inhibitory zone, which produced more than 15 mm clear zone for Eschericia coli and more than 24 mm clear zone for Staphylococcus aureus.
| Item Type: | Conference or Workshop Item (Paper) |
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| Subjects: | 500 Science > 540 Chemistry |
| Divisions: | Faculty of Agricultural Technology > Department of Food Technology |
| Depositing User: | Mas Teguh M. |
| Date Deposited: | 08 Sep 2015 21:18 |
| Last Modified: | 08 Sep 2015 21:18 |
| URI: | http://repository.unika.ac.id/id/eprint/1987 |
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