ADRIANTHO, AMADEUS (2024) ANALISIS CEMARAN MIKROORGANISME LABORATORIUM MIKROBIOLOGI DI FAKULTAS X MENGGUNAKAN METODE ENVIRONMENTAL MONITORING. S1 thesis, UNIVERSITAS KATOLIK SOEGIJAPRANATA SEMARANG.

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Abstract

Microbiology laboratory in X faculty is a new laboratory that is available for use by students majoring in Food Technology. The laboratory is still new, considering it was built in 2022. It was already operational but has not gone through any further testing about the laboratory’s cleanliness and level of microbial contamination. Microbial contamination observation is important because of how condusive the growth media is and how specific the target microorganism that is going to be studied. If the laboratory has a high level of microbial contamination, unwanted microorganism may contaminate and ruin the collected samples, making inaccuracies in the sample datas. One of the ways to measure microbial contamination is using environmental monitoring. Environmental monitoring could pinpoint the source of problems in food security and quality such as pathogens, allergens, spoilage organisms, or indicator organisms. Environmental monitoring could be applied to a laboratory to validate and verificate the effectivity of currently active cleaning programs. If it is deemed ineffective, environmental monitoring could also identify problems for future improvements. The resulting samples from this research could also be used as a baseline data for future studies or attempts to reduce microbial contamination. From the above considerations, there is a need to do environmental montoring in microbiology and fermentation laboratorium in X faculty to know the level of microbial contamination and consideration how to improve the laboratorium’s quality in the future. This study aims to identify what cleanroom class the microbiology lab in X faculty based on PerKa BPOM No 34 year 2018. The methods used in this study is passive air sampling-settle plate, surface sampling – contact plate, and surface sampling-swab. The tobserved parameters are average cfu/4 hours for passive air sampling-settle plate, and average cfu/plate for surface sampling – contact plate and surface sampling- swab. Passive air sampling – settle plate method resulted in an average of 71 cfu/4 hours, classifying it as D class. Surface samplingcontact plate method resulted in an average of 14 cfu/plate, classifying it as C class. Surface sampling – swab method resulted in an average of 25 cfu/plate, classifying it as C class. Based on these three methods, microbiology lab in faculty X classifies as D class, which is clean areas for low risk production

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