EVALUATION OF EFFECTIVENESS TWO BIOMARKERS (GLUTATHIONE AND LYSOSOMAL DESTABILIZATION)FOR COPPER AND CADMIUM EXPOSURES IN NILE TILAPIA (Oreochromis niloticus)

ENGELBERTA V. S., BENEDICTA CHRESTELLA (2016) EVALUATION OF EFFECTIVENESS TWO BIOMARKERS (GLUTATHIONE AND LYSOSOMAL DESTABILIZATION)FOR COPPER AND CADMIUM EXPOSURES IN NILE TILAPIA (Oreochromis niloticus). Other thesis, UNIKA SOEGIJAPRANATA.

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Abstract

Nile tilapia is one of the famous fish. Although Nile tilapia is freshwater fish, it is also cultivated in ponds along the north coastal areas of Java and usually located close to various industries that pose risk of contamination to water and fish. Heavy metals such as copper (Cu) and cadmium (Cd) is one class of contaminant that not only dangerous to the fish but also have a food safety implication. The consumption of contaminated fish may transfer these metals into human body and can cause negative health effects in the long term. To determine the severity of oxidative stress, the toxicity test is needed. For that, a number of biomarkers have been studied including reduced glutathione (GSH) and Lysosomal destabilization (LyD). These biomarkers are know to be sensitive to indicater metal contamination even in low concentration. In this case GSH and LyD are good candidates for effective early warning to Cu and Cd contamination in Nile tilapia. This study was started with fish acclimation in the laboratory water conditions prior to the toxicity test. It was then continued by determination of 96hr LC50 values. A series concentrations was used for biomarker evaluation, i.e. 1/20 of LC50, 1/10 of LC50, and 1/5 of LC50. Biomarkers evaluation were done by exposing the fish to the above metal concentrations for 20 days. Gill, liver, and muscles were disected for GSH measurement, while for LyD measurement liver was dissected. It was found thatGSH have a sensitivity and effectivity in gill and liverfor copper exposure, and in liver for cadmium exposure. LyD was effective at 1/10 of LC50 day 5 and 1/20 of LC50 day 5, respectively for Cu and Cd. Both biomarkers are effective in determining copper and cadmium exposures in Niletilapia even in low concentrations.

Item Type: Thesis (Other)
Subjects: > 660 Chemical engineering > Chemical Technology
> 660 Chemical engineering > Chemical Technology > Biotechnology
> 660 Chemical engineering > Food Technology
> 660 Chemical engineering > Food Technology > Food Contaminations
Divisions: Faculty of Agricultural Technology > Department of Food Technology
Depositing User: Mr Ignatius Eko Budi Setiono
Date Deposited: 30 Mar 2017 05:20
Last Modified: 30 Mar 2017 05:20
URI: http://repository.unika.ac.id/id/eprint/12951

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