YUARTA, TAN, JEFFRI WAN (2015) ISOLASI PROTEIN PADA KORO BENGUK, KORO PEDANG, KORO GLINDING DAN ANALISA PROFIL PROTEIN DENGAN METODE ELEKTROFORESIS. Other thesis, Prodi Teknologi Pangan Unika Soegijapranata.
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Abstract
Koro is one type of local beans that widespread in Indonesia. There are several types of koro with specific characteristics such as the shape and color of its beans. There are three types of koro that widely planted in Indonesia such as velvet beans, jack beans, and lima beans. Koro are high in protein and carbohydrate, but it has low fat content. Based on this condition, koro was a good raw material to be process become very useful product. Nowadays, koro only become a simple product because limited process applied, so we cannot get the maximum utilization of the raw material itself. This study aims to determine the protein contents, the most effective protein isolation method for each koro, and analysis of protein profiles in protein isolates. Koro that used in this research was velvet beans, jack beans, and lima beans. The isolates will be analyzed on protein content and protein profiles using electrophoresis. Based on preliminary test results, obtained drying protein isolate by freeze drying method showed optimal results of yield, isolate of velvet beans, isolate of jack beans, and isolate of lima beans respectively 79,326%, 17,962%, and 13,58%. Result of protein content showed that velvet beans surely have the highest protein content of 97,713%. Further isolate protein of jack beans is 70.897% and isolate protein of lima beans is 63%. Electrophoresis test (SDS-PAGE) was conducted to determine the protein profile of each protein isolate. Results of the test analyzed by bioinformatics method to determine the type of protein contained in the isolate. The results are 5 groups of proteins identified are fosfatase, levodopa, albumine, canavaline, legumine, concanavalin A, oleosin, lectin, chitinase, chymotripsin inhibitor, dan calmodulin.
| Item Type: | Thesis (Other) |
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| Subjects: | 600 Technology (Applied sciences) |
| Divisions: | Faculty of Agricultural Technology > Department of Food Technology |
| Depositing User: | Mrs Christiana Sundari |
| Date Deposited: | 14 Jan 2016 06:34 |
| Last Modified: | 14 Jan 2016 06:34 |
| URI: | http://repository.unika.ac.id/id/eprint/7340 |
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