Study of Proteins that Catalyze Silica formation and Polyunsaturated Fatty Acid synthesis in Marine Diatom Chaetoceros gracilis)

SUHARTONO, MAGGY THENAWIDJAJA and Pratiwi, Alberta Rika and Syah, Dahrul and Hardjito, Linawati Study of Proteins that Catalyze Silica formation and Polyunsaturated Fatty Acid synthesis in Marine Diatom Chaetoceros gracilis). In: 10th International Marine Biotechnology Conference. (Unpublished)

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Abstract

Silica polymers and polyunsaturated fatty acid (PUFA) are valuable substances due to their benefits for food and health industry. They are abundantly produced by diatom. The mechanism of their synthesis is controlled by several proteins. The aim of this research was to study the proteins involved in silica and PUFAs formation in Chaetoceros gracilis. Research methods include analysis of growth, cellular content of protein and lipid as well as fatty acid composition during growth. Protein characteristics were conducted by two-dimensional electrophoresis to estimate the molecular weight and isoelectric point. Proteins were identified by bioinformatic study. The cellular content of proteins, lipids and fatty acids were influenced by the growth phase. There were five silica proteins detected from silicaceous cell wall of this diatom. Silicic acid transport proteins which are active in the transport of silicic acid for silica synthesis was also identified during exponential to death growth phase. Eleven proteins involved in synthesis of PUFAs were identified, these included three microsomal desaturase proteins (Δ9 DES, Δ6 DES, ELO Δ6, prekursor ω-6 DES, prekursor ω-3 DES, spingolipid Δ8 DES, Δ12 DES, Δ4 DES, microsomal Δ4 DES, microsomal ω-6 DES and microsomal Δ6 DES).

Item Type: Conference or Workshop Item (Paper)
Subjects: 600 Technology (Applied sciences) > 660 Chemical engineering > 664 Food technology
Divisions: Faculty of Agricultural Technology > Department of Food Technology
Depositing User: Ms Rika Pratiwi
Date Deposited: 09 Jun 2022 06:58
Last Modified: 09 Jun 2022 06:58
URI: http://repository.unika.ac.id/id/eprint/28029

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