The Effect of Carbon and Nitrogen Supplementation on Bacteriocin Production of Lactic Acid Bacteria from Pickled Yellow Bamboo Shoots (Dendrocalamus Asper)

LINDAYANI, LINDAYANI and HARTAYANIE, LAKSMI (2018) The Effect of Carbon and Nitrogen Supplementation on Bacteriocin Production of Lactic Acid Bacteria from Pickled Yellow Bamboo Shoots (Dendrocalamus Asper). The Effect of Carbon and Nitrogen Supplementation on Bacteriocin Production of Lactic Acid Bacteria from Pickled Yellow Bamboo Shoots (Dendrocalamus Asper), 12 (1). ISSN 1978-3477

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Abstract

Six selected lactic acid bacteria (LAB) isolates from pickled Yellow Betung bamboo shoot were grown in the Mann Rogosa Sharpe-Broth (MRSB) media with different supplementation combination. The cell-free supermatant were evaluated for their ability to produce bacteriocin by adjusting its pH to 6.0 in order to remove organic acid effects. The bacteriocin activity was assayed by agar-well diffusion method. The inhibitory activity calculated in Activity Unit (AU in mm² ML⁴) of bacteriocins. The aimns of this paper is to explore the effect of different medium compositions on bacteriocin in production and its inhibitory activity againts pathogenic bacteria (Listeria monocytogenes FNCC 0156, Staphylococcus aureus FNCC 0047, and Escherichia coli FNCC 0091). Supplementations of carbon and nitrogen sources induced production of bacteriocins. LAB isolates grown in media without supplementation could not produce bacteriocins. Growth of isolate D44 in the presence of 2% of glucose and 2 % pf yeast extract yielded the largest bacteriocin inhibitory activity levels of 3179 AU mL⁴ against Listeria monocytogenes FNCC 0156, 4663 AU mL⁴ againts Staphylococcus aureus FNCC 0047, and 3109 AU mL⁴ against Escherichiacoli FNCC 0091

Item Type: Article
Subjects: 600 Technology (Applied sciences) > 660 Chemical engineering > 664 Food technology
Divisions: Faculty of Agricultural Technology > Department of Food Technology
Depositing User: ms F. Dewi Retnowati
Date Deposited: 02 Mar 2021 03:57
Last Modified: 02 Mar 2021 03:57
URI: http://repository.unika.ac.id/id/eprint/23703

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