EFFECT OF EXTRACTION TIME AND RATIO OF SAMPLESOLVENT ON ANTIBACTERIAL ACTIVITY AND TOTAL PHENOLIC COMPOUND OF Caulerpa lentilifera

HENDRYANTI, DEA NATHANIA (2015) EFFECT OF EXTRACTION TIME AND RATIO OF SAMPLESOLVENT ON ANTIBACTERIAL ACTIVITY AND TOTAL PHENOLIC COMPOUND OF Caulerpa lentilifera. Other thesis, PRODI TEKNOLOGI PANGAN UNIKA SOEGIJAPRANATA.

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Abstract

Seaweed are renewable living resource which has antibacterial potential. Nowdays, many researchers interested with Caulerpa lentilifera, a round seagrape which is also distributed in Indonesia (local name : Lato) but still poorly investigated in antibacterial activity. In fact, it has higher phenolic compound, which is act as an antibacterial agent in macroalgae, compared with almost all chlorophyta, phaeopyhta and rhodophyta species. The objectives of this research are to investigate the effect of extraction time (1, 2, 3 days) and ratio of sample-solvent (1:5, 1:10, 1:15 wv-1) in ethyl acetate extraction on antibacterial activity of dry C. lentilifera by using disk diffusion method followed by minimum inhibitory concentration (MIC) at 120, 60, 30, 15, and 7.5 μl and minimum bactericidal concentration (MBC) test against Gram-negative and Gram-positive bacteria. To determine the total phenolic compound to find out the relationship between antimicrobial activity and phenolic compound. The pathogenic bacteria used in this research are Staphylococcus aureus (FNCC 167), Escherichia coli (FNCC 194), Salmonella typhimurium (FNCC 0050) and Bacillus cereus (FNCC 0057). The antibacterial testing showed that inhibitory effect of C. lentilifera increased by increasing time of extraction and ratio of sample-solvent. The highest inhibitory action was achieved maximum level on 72 hours of extraction with ratio 1:15 wv-1. Caulerpa lentilifera has MIC value around 1.5 – 6 mg ml-1 which is classified as strong inhibitor and ratio of MBC : MIC value less than 4 which is considered as bactericidal agent against both Gram-negative and Gram-positive bacteria. Correlation analysis using SPSS showed very strong and very significant correlation between total phenolic compound and antibacterial activity with correlation coefficient is 0.594** and significant value is 0.001 at confident level of 95%. This correlation connection is directly proportional, means that the increasing amount of phenolic will followed by the increasing level of antibacterial activity of Caulerpa lentilifera. This research indicate that Caulerpa lentilifera possess a potential antibacterial activity against human pathogenic bacteria and one of its antibacterial agent is phenolic compound.

Item Type: Thesis (Other)
Subjects: > 660 Chemical engineering > Food Technology > Food Additives
Divisions: Faculty of Agricultural Technology > Department of Food Technology
Depositing User: Mrs Fransiska Septiana
Date Deposited: 26 Aug 2015 01:18
Last Modified: 26 Aug 2015 01:18
URI: http://repository.unika.ac.id/id/eprint/282

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